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  • 3T analytik
  • Gartenstraße 100
  • 78532 Tuttlingen
  • http://www.3t-analytik.de
  • Ansprechpartner
  • Gudrun Prepens
  • +49 (7461) 16503-11

Assessing indirect agent-induced gene toxicity

AUREA gTOXXs uncovers hidden DNA damaging potential of drugs, chemicals or natural products in human liver-derived cells

(PresseBox) (Tuttlingen, ) Genes are the blueprints for our height, eye color, personality or life expectancy, but also the likelihood of getting vascular disease, cancer or mental illness. Maintaining the integrity of the genome, that is the DNA, is thus of utmost importance. Reliable judgements on the effects of chemical or natural substances on DNA integrity are indispensable. All product ingredients are therefore subjected to ever-increasing control during product development, including the genotoxic risk assessment. The automated gTOXXs solution identifies the genotoxicity potential of liver metabolites in human liver-derived cells with high degree of scalability and certainty.

Notably, uncovering of genotoxicity is not always straightforward, but requires specific solutions. Some agents develop their toxic effects only when metabolized in particular organs; this is especially common in liver cells. These ingredients, initially considered harmless to the DNA turn out to be dangerous genotoxic substances. Not only drugs, but also food contaminants often develop toxicity by metabolic activation in the liver. In the case of aflatoxin B1, a toxin from widespread Aspergillus fungi, the detoxification mechanisms of the human liver work as an agitator: the aflatoxin B1 oxidation yields an extremely DNA reactive compound, which results in gene mutation. Aflatoxin metabolites are largely responsible for the rise of liver cancer worldwide.

For the in vitro genotoxicity risk assessment, the human-derived liver cell line, HepaRGTM is an excellent model for mapping the metabolic response of human hepatocytes (1, 2). HepaRG™ cells naturally lend themselves to OECD validated in vitro genotoxicity testing. In a recent study, 3T has shown that AUREA gTOXXs reliably detects DNA strand breaks in HepaRGTM cells that have been exposed to aflatoxin B1 (3). With the combination of the HepaRGTM cell line and the AUREA gTOXXs solution, 3T GmbH & Co.KG is breaking new ground in in vitro 'Genotox' risk assessment. Importantly, the risk assessment of DNA strand break over time by gTOXXs testing reports not only on the DNA damage, but also the repair capacity. This unique feature provides valuable insights into the physiological state of the HepaRGTM cells.

The rapid risk assessment of the genotoxic potency of pro-toxicants at an early stage of your product development by AUREA gTOXXs with HepaRGTM yields several valuable advantages:
  • Fast and standardized procedures of risk assessment using the human hepatocyte cell line HepaRGTM
  • High kinetic resolution of DNA damage and repair activity
  • Costs and time savings in the early stages of product development and scientific research
Conclusion: The automated gTOXXs solution differentiates the risk potential of liver metabolites in HepaRGTM with high degree of scalability and certainty with fast, reliable and robust results.

Safe and increasingly animal-free products can be developed thanks to risk assessment by AUREA gTOXXs!  
  1. Wang S, Wang M et al. 2018. Toxins (Basel) 10 pii E154.
  2. Murayama N, Yamazaki H. 2018. J Toxicol Sci, 43:241-245
  3. Dressler D, Pfitzner I et al. 2018. SOT 57th Meet ToXExpo, San Antonio, March 11th -15th

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3T analytik

3T GmbH & Co.KG located in Tuttlingen, Germany, offers solutions for high throughput genotoxicity screening, QCM-D based surface interaction analysis, high end lab automation technology and microfabrication. The instruments from 3T ensure innovative and reliable technology with attention to the ease of use for established and emerging industrial and academic applications.