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pDG Helper & Packaging plasmids for AAV production now exclusively available from PlasmidFactory

(PresseBox) (Bielefeld, ) PlasmidFactory has just received a worldwide exclusive license from the German Cancer Research Center (DKFZ) for manufacturing, distribution and use of the Helper & Packaging Plasmids from the pDG- respectively pDPfamily. "We have put the marketing of this system in the hands of our longterm cooperation partner. As an established supplier of research labs as well as biotech and pharmaceutical companies around the world, PlasmidFactory guarantees worldwide use of our advanced technology." explains Professor Dr. Jürgen Kleinschmidt from the department of tumor virology at DKFZ. The pDG system for the production of adenoassociated viruses (AAV) is a twoplasmid system. This means that in contrast to the tripletransfection with three different plasmids, only two components are needed: a plasmid with the gene to be transferred and the corresponding pDG plasmid that includes all the information for the growth and packaging of AAV vector plasmids (the socalled "helper & packaging" functions). An additional infection with helper viruses - as required with the original systems - is not necessary. "The pDG system is currently both in technical and economic terms the world's most efficient system for plasmid based AAV production. The plasmid production costs are reduced dramatically by the elimination of the third plasmid," says Dr. Martin Schleef, CEO of PlasmidFactory." The use of AAV vectors in gene therapy is currently increasing significantly and the pDGsystem makes the production process for these vectors much easier "adds Dr. Marco Schmeer, Product Manager R & D, PlasmidFactory. The pDG plasmids are manufactured in reproducible quality by using PlasmidFactory's standardized and controlled process. Through a comprehensive analysis a defined homogeneous product is guaranteed. All plasmids are fully characterized, including LAL test (LPSendotoxin), BCA test (total protein), UV spectroscopy, restriction analysis and the detection of RNA and chromosomal DNA with AGE. "The preparation of pDG plasmid DNA in qualities for GMP production of AAV is another integral part of our services," adds Dr. Schleef.

The plasmids of the pDG family for the production of AAV are available in different serotypes and are immediately available from PlasmidFactory's In Stock service at any time.

About DKFZ

The German Cancer Research Center (Deutsches Krebsforschungszentrum, DKFZ) is the largest biomedical research institute in Germany and is a member of the Helmholtz Association of National Research Centers. More than 2,000 staff members, including 850 scientists, are investigating the mechanisms of cancer and are working to identify cancer risk factors. They provide the foundations for developing novel approaches in the prevention, diagnosis, and treatment of cancer. In addition, the staff of the Cancer Information Service (KID) offers information about the widespread disease of cancer for patients, their families, and the general public. The Center is funded by the German Federal Ministry of Education and Research (90%) and the State of Baden-Württemberg (10%).

About PlasmidFactory GmbH & Co. KG

PlasmidFactory is relieving the scientist of the daily routine tasks. In its laboratories the company is producing highquality plasmid DNA meeting the individual requirements of the research scientist.

Besides contract manufacturing of plasmid DNA and minicircle DNA, PlasmidFactory is also offering genesynthesis and geneoptimisation. The socalled "In Stock products‟ are complementing the product portfolio: these plasmids are always in stock and are usually shipped within 48 hours of order. Presently the following In Stock products are available: various reportergene plasmids and minicircles, pDG plasmids for the AAV virus production and pEPI plasmids with S/MAR elements.

PlasmidFactory‟s research and development as well as its complete service branches are based in Germany. With various projects sponsored by the German Federal Ministry of Education and Research (BMBF) plus European research cooperations, PlasmidFactory is the driving force in the development of gene vectors. Respectively the company is working in close cooperation with many renowned institutions such as the German Cancer Research Institute (DKFZ) in Heidelberg, the University of Munich, the Charité Berlin and the Institut Pasteur to name just a few.

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